概述

  • 产品名称Anti-C9orf169抗体
    参阅全部 C9orf169 一抗
  • 描述
    兔多克隆抗体to C9orf169
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant Protein Epitope Signature Tag: PIQNQQAWQQ PGNPYSSSQR QAGLTYAGPP PVGRGDDIAH HCCCCPCCHC CHCPPFCRCH SCCCCVIS, corresponding to amino acids 77 - 144 of Human C9orf169.

  • 阳性对照
    • Human esophagus tissue
      Over-expression HEK293T cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

Anti-C9orf169 antibody 图像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121701 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121701, at a 1/225 dilution, staining C9orf169 in paraffin-embedded Human esophagus tissue by Immunohistochemistry.
  • All lanes : Anti-C9orf169 antibody (ab121701) at 1/250 dilution

    Lane 1 : Blank vector transfected HEK293T cell lysate
    Lane 2 : Over-expression HEK293T cell lysate, with a C terminal myc-DDK tag

Anti-C9orf169 antibody (ab121701)参考文献

ab121701 has not yet been referenced specifically in any publications.

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