概述

  • 产品名称
  • 描述
    兔多克隆抗体to C9
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human C9.

    (Peptide available as ab94838.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human placenta; Human plasma total protein

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab67040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 63 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
    • 组织特异性
      Plasma.
    • 疾病相关
      Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
    • 序列相似性
      Belongs to the complement C6/C7/C8/C9 family.
      Contains 1 EGF-like domain.
      Contains 1 LDL-receptor class A domain.
      Contains 1 MACPF domain.
      Contains 1 TSP type-1 domain.
    • 翻译后修饰
      Thrombin cleaves factor C9 to produce C9a and C9b.
      Phosphorylation sites are present in the extracelllular medium.
    • 细胞定位
      Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ARMD15 antibody
      • C9 antibody
      • C9 deficiency antibody
      • C9 deficiency with dermatomyositis antibody
      • C9D antibody
      • CO9_HUMAN antibody
      • Complement component 9 antibody
      • Complement component 9 deficiency antibody
      • Complement component C9 antibody
      • Complement component C9b antibody
      see all

    图片

    • All lanes : Anti-C9 antibody (ab67040) at 1 µg/ml

      Lane 1 : Human Plasma Total Protein Lysate
      Lane 2 : Human placenta tissue lysate - total protein (ab29745)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 63 kDa
      Observed band size : 74 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 16 minutes

    实验方案

    文献

    ab67040 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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