概述

  • 产品名称Anti-C9抗体
    参阅全部 C9 一抗
  • 描述
    兔多克隆抗体to C9
  • 特异性This product may also detect C9 within the C5b-9 complex, but we have no evidence to show this. Any feedback from our customers is welcome. We recommend ab55811 for specifically detecting the C5b-9 complex.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human C9.

    (Peptide available as ab71328.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Kidney (Human) Placenta (Human)

性能

应用

Our Abpromise guarantee covers the use of ab64011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa).

靶标

  • 功能Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
  • 组织特异性Plasma.
  • 疾病相关Defects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
  • 序列相似性Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 EGF-like domain.
    Contains 1 LDL-receptor class A domain.
    Contains 1 MACPF domain.
    Contains 1 TSP type-1 domain.
  • 翻译后修饰Thrombin cleaves factor C9 to produce C9a and C9b.
    Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位Secreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C9 antibody
    • C9 deficiency antibody
    • C9 deficiency with dermatomyositis antibody
    • CO9_HUMAN antibody
    • Complement component 9 antibody
    • Complement component 9 deficiency antibody
    • Complement component C9 antibody
    • Complement component C9b antibody
    see all

Anti-C9 antibody 图像

  • All lanes : Anti-C9 antibody (ab64011) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 40,70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes

Anti-C9 antibody (ab64011)参考文献

This product has been referenced in:
  • Papareddy P  et al. C-terminal Peptides of Tissue Factor Pathway Inhibitor Are Novel Host Defense Molecules. J Biol Chem 285:28387-98 (2010). WB . Read more (PubMed: 20592020) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry.

I can confirm that both antibodies may also detect C9 within the C5b-9 complex, but we have no evidence to show this. Any feedback from our customers is welcome.

Both antibodieswere raised against an immu...

Read More

Thank you for your enquiry. The reason that the molecular weight is different could be that the C5-b-9 complex degrades under proteolysis and therefore on a western blot, we are only seeing degradation products. In this case, it is the phosphoryla...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"