antigen sequence: ANIPGYTGKV HFTATHPANS NIPSTTPSPD SELHRVFQKE MAVDLFRHQA PLSRLVTTVR PYNPFNKKDK ETIDY, corresponding to amino acids 364-438 of Human C7orf72
Human skeletal muscle tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Information by UniProt
Chromosome 7 open reading frame 72 antibody
Uncharacterized protein C7orf72 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C7orf72 antibody (ab121863)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, nuclear membrane, cytoplasm and cytoskeleton (actin filaments). Recommended concentration of ab121863 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C7orf72 antibody (ab121863)
ab121863, at a 1/10 dilution, staining C7orf72 in paraffin-embedded Human skeletal muscle tissue by immunohistochemistry.
has not yet been referenced specifically in any publications.
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