Synthetic peptide within residues: YLVLWAVRKH LRRLYRRQER HRRHHVRCHA APRPNPAQSL KLDAQSPL, corresponding to C terminal amino acids 159-208 of Human C7ORF61 (NP_001004323)
- Human fetal brain lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
ab84941 is purified by a peptide affinity chromatography method.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 24 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
The exact function of C7ORF61 remains unknown.
- Chromosome 7 open reading frame 61 antibody
- Hypothetical protein LOC402573 antibody
Western blot - C7ORF61 antibody (ab84941)
Anti-C7ORF61 antibody (ab84941) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µgSecondary
anti-Rabbit IgG HRP at 1/50000 dilutionPredicted band size:
24 kDaObserved band size:
31 kDa (why is the actual band size different from the predicted?
Gel concentration 12%
has not yet been referenced specifically in any publications.
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