antigen sequence, corresponding to N terminal amino acids 3-61 (HSSQDTGSCG IQEDGKLYVV DSINDLNKLN LCPAGSQHLF PLEDKIPVLG TNSGNGSRS) of Human C7orf53.
- Human kidney tissue. Transfected HEK293T cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Information by UniProt
- C7orf53 antibody
- CG053_HUMAN antibody
- Chromosome 7 open reading frame 53 antibody
- Coiled-coil domain-containing transmembrane protein C7orf53 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C7orf53 antibody (ab121406)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121406 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C7orf53 antibody (ab121406)
ab121406 at 1/20 dilution staining C7orf53 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Western blot - Anti-C7orf53 antibody (ab121406)
All lanes : Anti-C7orf53 antibody (ab121406) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : C7orf53-transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"