antigen sequence: LKHLKSITPP SHVVDLLDSI EDMDLCHVVP AEKKIDEAKD ERLCENNAEF NKNCSKSHSG IDCSYVECCR TQEHAHSENP SPT, corresponding to amino acids 118-200 of Human C7orf36.
Human cerebral cortex tissue; HEK293T cells transfected with C7orf36.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/500.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Immunocytochemistry/ Immunofluorescence - Anti-C7orf36 antibody (ab121595)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm and mitochondria. Recommended concentration of ab121595 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C7orf36 antibody (ab121595)
ab121595 at 1/50 dilution staining C7orf36 in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
Western blot - Anti-C7orf36 antibody (ab121595)
All lanes : Anti-C7orf36 antibody (ab121595) at 1/250 dilution Lane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
has not yet been referenced specifically in any publications.
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