antigen sequence: GPGERERATV LIKRINVVPD QPSERALRLV ASSKINSRSL TIFGTGDTLK AITMTANSGF VRAANNQGVK FSVFIHQPRA LTESKEALAT PLPKDYTTD, corresponding to amino acids 320-418 of Human C7orf25.
- Human urinary bladder tissue; Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
Anti-C7orf25 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-C7orf25 antibody (ab121621)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121621 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C7orf25 antibody (ab121621)
ab121621 at 1/300 staining C7orf25 in Paraffin Embedded Human urinary bladder tissue by Immunohistochemistry.
Note: strong nuclear positivity in urothelial cells.
Anti-C7orf25 antibody (ab121621)参考文献
has not yet been referenced specifically in any publications.
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