概述

  • 产品名称Anti-C6orf58抗体
    参阅全部 C6orf58 一抗
  • 描述
    兔多克隆抗体to C6orf58
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 212-241 of Human C6orf58 (NP_001010905.1), conjugated to KLH.

  • 阳性对照
    • K-562 cell line lysates This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab107392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 38 kDa.

靶标

Anti-C6orf58 antibody 图像

  • ICC/IF image of ab107392 stained HeLa cells. The cells were 4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107392 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Dylight® 488 goat anti- rabbit (ab96899) IgG (H+L) preadsorbed, used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-C6orf58 antibody (ab107392) at 1/100 dilution + K-562 cell line lysates at 35 µg

    Predicted band size : 38 kDa

Anti-C6orf58 antibody (ab107392)参考文献

ab107392 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab107392.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"