antigen sequence: SFRRAFYHKK HTSKEPRRAG AAGAASPEAR RPKRPSFLPL CVGGHRPSTS SSLDPEDLEC REPLPAEGEP VVISEAPSQ, corresponding to internal sequence amino acids 438-516 of Human C6orf222.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Information by UniProt
- C6orf222 antibody
- CF222_HUMAN antibody
- Chromosome 6 open reading frame 222 antibody
- Uncharacterized protein C6orf222 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C6orf222 antibody (ab122471)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122471 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C6orf222 antibody (ab122471)
ab122471, at 1/200 dilution, staining C6orf222 in paraffin-embedded Human testis tissue by Immunohistochemitry.
has not yet been referenced specifically in any publications.
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