概述

  • 产品名称Anti-C6orf211抗体
    参阅全部 C6orf211 一抗
  • 描述
    兔多克隆抗体to C6orf211
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 52-172 of Human C6orf211.

  • 阳性对照
    • Human testis tissue; HEK293T Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20ºC.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab121158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-C6orf211 antibody 图像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and cytoplasm. Recommended concentration of ab121158 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121158 at 1/50 dilution staining C6orf211 in Paraffin-emebedded Human Testis tissue by Immunohistochemistry.

    Note: strong cytoplasmic positivity in cells in seminiferus ducts.
  • All lanes : Anti-C6orf211 antibody (ab121158) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : HEK293T Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

Anti-C6orf211 antibody (ab121158)参考文献

ab121158 has not yet been referenced specifically in any publications.

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