Anti-CENP-W抗体- N-terminal (ab189957)
Key features and details
- Rabbit polyclonal to CENP-W - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CENP-W抗体- N-terminal -
描述
兔多克隆抗体to CENP-W - N-terminal -
宿主
Rabbit -
特异性
At least three isoforms of CENP-W are known to exist; ab189957 will detect all three isoforms. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human CENP-W (N terminal). The peptide corresponds to 16 amino acids.
Database link: Q5EE01 -
阳性对照
- Human spleen tissue. HeLa cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab189957于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 10 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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说明 |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 10 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
靶标
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功能
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation (By similarity). The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres (By similarity). Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPW has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis. -
组织特异性
Highly expressed in ovary, liver, lung and pancreas and to a lower extent in breast and gastrointestinal tract cancers; such as those of the colon, rectum and stomach. Overexpressed in high grade breast invasive tumors. Expressed in many cancer cell types. -
序列相似性
Belongs to the CENPW family. -
细胞定位
Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Nucleus matrix. Nucleus > nucleolus. Constitutively localizes to centromeres throughout the cell cycle, and to the inner kinetochore during mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 387103 Human
- Omim: 611264 Human
- SwissProt: Q5EE01 Human
- Unigene: 486401 Human
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别名
- C6ORF173 antibody
- cancer upregulated gene 2 antibody
- Cancer-up-regulated gene 2 protein antibody
see all
图片
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Lane 1 : Anti-CENP-W antibody - N-terminal (ab189957) at 0.5 µg/ml
Lane 2 : Anti-CENP-W antibody - N-terminal (ab189957) at 1 µg/ml
All lanes : HeLa cell lysate
Predicted band size: 10 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human spleen sections labeling CENP-W with ab189957 at 5 µg/ml.
数据表及文件
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Datasheet download
文献 (0)
ab189957 尚未被引用在任何文献中。