概述

  • 产品名称Anti-C5R1抗体
    参阅全部 C5R1 一抗
  • 描述
    兔多克隆抗体to C5R1
  • 特异性This antibody detects endogenous levels of total C5R1 protein.
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthesized non-phosphopeptide derived from human C5R1 around the phosphorylation site of serine 338.

  • 阳性对照
    • WB: Extracts from HeLa cells treated with PMA (125ng/ml, 30mins).

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab59390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
IHC-P
  • 应用说明ELISA: 1/5000.
    IHC-P: Use at a concentration of 1 µg/ml.
    WB: 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 39 kDa).
    Lysis buffer: 50mM Tris(pH7.4),150mM NaCl,1% Triton X-100,1% sodium deoxycholate,0.1% SDS and sodium orthovanadate,sodium fluoride,EDTA ,leupeptin.
    Blocking buffer: 3%BSA, 2 hours. Dilute the primary antibody in blocking buffer.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
    • 序列相似性Belongs to the G-protein coupled receptor 1 family.
    • 翻译后修饰Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ANAPHYLATOXIN RECEPTOR antibody
      • C5a anaphylatoxin chemotactic receptor antibody
      • C5a anaphylatoxin receptor antibody
      • C5A antibody
      • C5a ligand antibody
      • C5a-R antibody
      • C5aR antibody
      • C5AR_HUMAN antibody
      • c5ar1 antibody
      • C5R1 antibody
      • CD88 antibody
      • CD88 antigen antibody
      • Complement component 5 receptor 1 antibody
      • Complement component 5 receptor 2 antibody
      • Complement component 5a receptor 1 antibody
      see all

    Anti-C5R1 antibody 图像

    • All lanes : Anti-C5R1 antibody (ab59390) at 1/500 dilution

      Lane 1 : Extracts from HeLa cells, treated with PMA (125ng/ml, 30mins).
      Lane 2 : Extracts from HeLa cells, treated with PMA (125ng/ml, 30mins) plus peptide.


      Predicted band size : 39 kDa
      Observed band size : 46 kDa (why is the actual band size different from the predicted?)
    • ab59390 staining C5R1 in human lung.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    Anti-C5R1 antibody (ab59390)参考文献

    This product has been referenced in:
    • Zhang C  et al. Antagonist of C5aR Prevents Cardiac Remodeling in Angiotensin II-Induced Hypertension. Am J Hypertens N/A:N/A (2014). Mouse . Read more (PubMed: 24419904) »

    See 1 Publication for this product

    Product Wall

    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (APRE19 cell line)
    Loading amount 20 µg
    Specification APRE19 cell line
    Gel Running Conditions Reduced Denaturing (10% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. lei wang

    Verified customer

    提交于 Aug 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"