Anti-C5b-9抗体[aE11] (ab66768)

概述

  • 产品名称
    Anti-C5b-9抗体[aE11]
    参阅全部 C5b-9 一抗
  • 描述
    小鼠单克隆抗体[aE11] to C5b-9
  • 经测试应用
    适用于: IHC-P, Flow Cyt, ICC/IF, ELISA, IHC-Frmore details
    不适用于: WB
  • 种属反应性
    与反应: Horse, Human, Pig, Monkey, Baboon
  • 免疫原

    Full length protein corresponding to Human C5b-9.

  • 表位
    ab66768 binds to the neoepitope exposed on C9 when incorporated into TCC (terminal complement complex).

性能

应用

Our Abpromise guarantee covers the use of ab66768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 23803904

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 20688727
ELISA Use at an assay dependent concentration.
IHC-Fr 1/10.
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 相关性
      Activation of the complement system plays a key role in normal inflammatory response to injury but may cause substantial injury when activated inappropriately. The complement system is activated either through the classical (antibody induced) or the alternative (microbial surface, polysacharride induced) pathway, both leading to the formation of the C5b9 complex. Fluid phase binding of the multifunctional glycoprotein S protein (vitronectin) to C5b9 leads to the formation of a cytolytically inactive complex, SC5b9, which is unable to attach to cells.
    • 细胞定位
      Secreted
    • 数据库链接
    • 别名
      • C5 antibody
      • c5b 9 antibody
      • C6 antibody
      • C7 antibody
      • C8 antibody
      • C9 antibody
      • Complement component 5 antibody
      • Complement component 6 antibody
      • Complement component 7 antibody
      • Complement component 8 antibody
      • Complement component 9 antibody
      see all

    Anti-C5b-9 antibody [aE11] 图像

    • ab66768 staining C5b-9 (red) in porcine liver tissue by Immunohistochemistry (Frozen sections).

      Tissue was taken from allotransplants of wild-type or a1,3-galactosyltransferase gene-knockout (GTKO) pig liver, 2 hours after grafting or at necroscopy (nec). Tissue was fixed with paraformaldehyde and blocked with 20% donkey serum for 1 hour at room temperature, before being incubated with primary antibody (1/100 in diluent).
    • ab66768 staining C5b-9 in monkey retina cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton-X and blocked with 4% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody. Counterstained with DAPI. Magnification: 20X.

      See Abreview

    • Immunohistochemical analysis of frozen tonsil tissue section labelling C5b-9 with ab66768 at a dilution of 1/10. Vascular endothelia and dendritic cells of germinal center have strong and intensive label.Anti-Complement component C5b-9 (human)

       

       

    Anti-C5b-9 antibody [aE11] (ab66768)参考文献

    This product has been referenced in:
    • Mohan RR  et al. Peptide redesign for inhibition of the complement system: Targeting age-related macular degeneration. Mol Vis 22:1280-1290 (2016). Read more (PubMed: 27829783) »
    • Nakamura A  et al. Initial pulmonary respiration causes massive diaphragm damage and hyper-CKemia in Duchenne muscular dystrophy dog. Sci Rep 3:2183 (2013). Read more (PubMed: 23851606) »

    See all 8 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
    Sample
    Monkey Tissue sections (Retina)
    Specification
    Retina
    Permeabilization
    Yes - 0.5 Triton-X
    Fixative
    Paraformaldehyde
    Username

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    Verified customer

    提交于 Jun 10 2014

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