概述

  • 产品名称Anti-C5抗体
    参阅全部 C5 一抗
  • 描述
    兔多克隆抗体to C5
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human C5.

    (Peptide available as ab69125.)

  • 阳性对照
    • This antibody gave a positive signal in Human Plasma Total Protein. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

性能

应用

Our Abpromise guarantee covers the use of ab66850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 188, 111 kDa (predicted molecular weight: 188 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
    Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
  • 疾病相关Defects in C5 are the cause of complement component 5 deficiency (C5D) [MIM:609536]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
    Note=An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
  • 序列相似性Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Anaphylatoxin C5a analog antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody
    • C5 antibody
    • C5a anaphylatoxin antibody
    • C5a antibody
    • C5b antibody
    • CO5_HUMAN antibody
    • Complement C5 alpha'' chain antibody
    • Complement C5 antibody
    • Complement component C5 antibody
    • CPAMD4 antibody
    • prepro-C5 antibody
    see all

Anti-C5 antibody 图像

  • IHC image of C5 antibody staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66850, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-C5 antibody (ab66850) at 1 µg/ml

    Lane 1 : Human Plasma Total Protein Lysate at 5 µg
    Lane 2 : Human Plasma Total Protein Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 188 kDa
    Observed band size : 111,188 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 220 kDa (possible glycosylated form),33 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes
    We hypothesis that the observed 188 kDa band represents the whole C5 protein, whereas the 111 kDa band represents the C5 alpha chain. The 220 kDa reaction could signify the glycosylated mature protein.
  • ICC/IF image of ab66850 stained MCF7 cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66850, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-C5 antibody (ab66850)参考文献

This product has been referenced in:
  • Shen H  et al. Increased activity of the complement system in the liver of patients with alcoholic hepatitis. Exp Mol Pathol 97:338-44 (2014). Human . Read more (PubMed: 25217811) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry. This immunogen covers the c-terminal end of the complement beta chain, all of the pro-peptide part in between and the n-terminal end of the complement C5 alpha chain). This antibody should therefore recognise the who...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"