概述

  • 产品名称Anti-C5 / C5a抗体
    参阅全部 C5 / C5a 一抗
  • 描述
    兔多克隆抗体to C5 / C5a
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human C5/ C5a aa 1450-1550 conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab46167)

  • 阳性对照
    • This antibody gave a positive signal in purified human Complement C5 protein.
  • 常规说明


    This antibody binds specifically to the alpha / alpha' chain of C5, which forms part of C5b

性能

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应用

Our Abpromise guarantee covers the use of ab46168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 188 kDa).Can be blocked with C5 / C5b peptide (ab46167).

靶标

  • 功能Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
    Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
  • 疾病相关Complement component 5 deficiency
    An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
  • 序列相似性Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Anaphylatoxin C5a analog antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody
    • C5 antibody
    • C5a antibody
    • CO5_HUMAN antibody
    • Complement C5 alpha'' chain antibody
    • Complement C5 antibody
    • Complement component C5 antibody
    • CPAMD4 antibody
    see all

Anti-C5 / C5a antibody 图像



  • Predicted band size : 188 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: C5 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: A549 whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab46168 observed at 170 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab46168 was shown to recognize C5 when C5 knockout samples were used, along with additional cross-reactive bands. Wild-type and C5 knockout samples were subjected to SDS-PAGE. Ab46168 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10003 dilution for 1 hour at room temperature before imaging.

  • Anti-C5 / C5a antibody (ab46168) at 1 µg/ml + Human Complement C5 Protein at 0.1 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 188 kDa
    Observed band size : 115 kDa (why is the actual band size different from the predicted?)

    Complement component C5 protein is comprised of an alpha and beta chain which have predicted molecular weights of 115 kDa and 75 kDa respectively.  ab46168 specifically detects the C5 alpha chain.

Anti-C5 / C5a antibody (ab46168)参考文献

ab46168 has not yet been referenced specifically in any publications.

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