antigen sequence: LMRLEEYKKE QAINRAGIVQ EDVQPPGLKV WSDPFGRK, corresponding to amino acids 131-168 of Human C4orf52.
- Human duodenum tissue; RT 4 and U 251 MG cell lysates; Human Liver lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Information by UniProt
- C4orf52 antibody
- CD052_HUMAN antibody
- Chromosome 4 open reading frame 52 antibody
- Uncharacterized protein C4orf52 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C4orf52 antibody (ab121500)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus, nucleoli and cytoplasm. Recommended concentration of ab121500 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4orf52 antibody (ab121500)
ab121500, at 1/75 dilution, staining C4orf52 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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