antigen sequence: MEVDAPGVDG RDGLRERRGF SEGGRQNFDV RPQSGANGLP KH, corresponding to amino acids 1-42 of Human C4orf3.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Information by UniProt
- C4orf3 antibody
- CD003_HUMAN antibody
- Chromosome 4 open reading frame 3 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C4orf3 antibody (ab121901)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and cytoplasm. Recommended concentration of ab121901 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4orf3 antibody (ab121901)
ab121901, at 1/1000 dilution, staining C4orf3 in paraffin embedded Human duodenum tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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