概述

  • 产品名称Anti-C4b抗体
    参阅全部 C4b 一抗
  • 描述
    小鼠单克隆抗体to C4b
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human, Chimpanzee
  • 免疫原

    Recombinant fragment: NNRQIRGLEE ELQFSLGSKI NVKVGGNSKG TLKVLRTYNV LDMKNTTCQD LQIEVTVKGH VEYTMEANED YEDYEYDELP AKDDPDAPLQ PVTPLQLFEG, corresponding to amino acids 1347-1447 of Human C4b (NP_000583) with tag.

  • 阳性对照
    • Recombinant tagged human C4b fragment.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2b
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 193 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
      Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    • 序列相似性Contains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • 翻译后修饰Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Basic complement C4 antibody
      • C2B5 antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
      • C4b antibody
      • C4B1 antibody
      • C4B2 antibody
      • C4B3 antibody
      • CO4B_HUMAN antibody
      • Complement C4 gamma chain antibody
      • Complement component 4B antibody
      • CPAMD3 antibody
      see all

    Anti-C4b antibody 图像

    • Anti-C4b antibody (ab76504) at 1 µg/ml + Recombinant tagged human C4b fragment at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 193 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    Anti-C4b antibody (ab76504)参考文献

    ab76504 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"