概述

  • 产品名称Anti-C4b抗体
    参阅全部 C4b 一抗
  • 描述
    鸡多克隆抗体to C4b
  • 经测试应用适用于: WB, ELISA, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified Human C4b protein.

  • 阳性对照
    • Human Placenta tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 克隆多克隆
  • 同种型IgY
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab118887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 193 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 0.1µg for 106 cells. ab37382-Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.

靶标

  • 功能C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • 序列相似性Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • 翻译后修饰Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Basic complement C4 antibody
    • C2B5 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
    • C4b antibody
    • C4B1 antibody
    • C4B2 antibody
    • C4B3 antibody
    • CO4B_HUMAN antibody
    • Complement C4 gamma chain antibody
    • Complement component 4B antibody
    • CPAMD3 antibody
    see all

Anti-C4b antibody 图像

  • ab118887, at 20 µg/ml, staining C4b in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-C4b antibody (ab118887)参考文献

ab118887 has not yet been referenced specifically in any publications.

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