Anti-C4 binding protein抗体(ab8788)

概述

  • 产品名称Anti-C4 binding protein抗体
    参阅全部 C4 binding protein 一抗
  • 描述
    羊多克隆抗体to C4 binding protein
  • 特异性This product gives a single arc when tested by IEP against fresh human plasma. Identity has been confirmed by double diffusion (Ouchterlony) vs fresh human plasma and a known anti human C4BP.
  • 经测试应用适用于: WB, Flow Cyt, Double Immunodiffusion, Immunoelectrophoresis, Othermore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human C4BP purified from plasma and shown to be homogeneous by SDS-PAGE.

  • 常规说明


    C4-binding protein (C4BP) is a multimeric protein, synthesised in the liver. The most abundant form consists of seven alpha-chains (70kDa) and one beta-chain (45kDa) all linked by disulphide bonds to form the native protein with a molecular weight of 570kDa. It is is a plasma protein with a concentration of about 150mg/L in normal plasma. It is a component of the classical complement pathway. C4BP down regulates complement activity in two ways, it binds to C4b thus inhibiting the formation of C3-convertase (C4bC3a) and it accelerates the decay of existing convertases. C4BP acts as a cofactor in Factor I mediated C4b proteolysis. C4BP may also have a regulatory role in the coagulation system, by binding to Protein S. When bound toC4BP, Protein S is inactive.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: Glycine buffered saline, 0.1% EACA, 0.01% Benzamidine, 1mM EDTA, pH 7.4
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Antiserum is prepared by immunisation of sheep with human C4BP and, if necessary, adsorbed tomonospecificity by use of solid-phase adsorbents. An immunoglobulin fraction is then produced. The titre is adjusted so that inter-batch variation is within 10%. The productis 0.2µm filtered.
  • Primary antibody说明C4-binding protein (C4BP) is a multimeric protein, synthesised in the liver. The most abundant form consists of seven alpha-chains (70kDa) and one beta-chain (45kDa) all linked by disulphide bonds to form the native protein with a molecular weight of 570kDa. It is is a plasma protein with a concentration of about 150mg/L in normal plasma. It is a component of the classical complement pathway. C4BP down regulates complement activity in two ways, it binds to C4b thus inhibiting the formation of C3-convertase (C4bC3a) and it accelerates the decay of existing convertases. C4BP acts as a cofactor in Factor I mediated C4b proteolysis. C4BP may also have a regulatory role in the coagulation system, by binding to Protein S. When bound toC4BP, Protein S is inactive.
  • 克隆多克隆
  • 同种型IgG
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab37385-Sheep polyclonal IgG, is suitable for use as an isotype control with this antibody.
Double Immunodiffusion Use at an assay dependent concentration.
Immunoelectrophoresis Use at an assay dependent concentration.
Other Use at an assay dependent concentration.

靶标

  • 功能Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S.
  • 序列相似性Contains 3 Sushi (CCP/SCR) domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C4b binding protein beta chain antibody
    • C4b-binding protein beta chain antibody
    • C4BP antibody
    • C4BPB antibody
    • C4BPB_HUMAN antibody
    • Complement component 4 binding protein beta antibody
    • Complement component 4 binding protein beta chain antibody
    • Complement component 4-binding protein beta antibody
    • OTTHUMP00000034288 antibody
    • OTTHUMP00000034289 antibody
    • OTTHUMP00000034375 antibody
    • OTTHUMP0000003474 antibody
    • Proline rich protein antibody
    • PRP antibody
    see all

Anti-C4 binding protein antibody 图像

  • Image from Pham CT et al, J Biol Chem. 2011 Jan 7;286(1):123-30. Epub 2010 Nov 3, Fig 8. DOI 10.1074/jbc.M110.180760

Anti-C4 binding protein antibody (ab8788)参考文献

This product has been referenced in:
  • Kuznetsova NR & Vodovozova EL Differential binding of plasma proteins by liposomes loaded with lipophilic prodrugs of methotrexate and melphalan in the bilayer. Biochemistry (Mosc) 79:797-804 (2014). WB ; Human . Read more (PubMed: 25365489) »
  • Pham CT  et al. Variable Antibody-dependent Activation of Complement by Functionalized Phospholipid Nanoparticle Surfaces. J Biol Chem 286:123-30 (2011). WB . Read more (PubMed: 21047788) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry regarding ab8788.

This product has only been tested in human samples and the cross-reactivity with other species has not been determined yet.

According to Blast search, the sequence homology of C4b-binding...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Cell Lysate and supernatant of CHO cell cultures)
Loading amount 100000 cells
Specification Cell Lysate and supernatant of CHO cell cultures
Gel Running Conditions Non-reduced Non-Denaturing (Native) (8% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Dec 03 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (serum)
Specification serum
Permeabilization No
Username

Dr. jose yuste

Verified customer

提交于 Feb 17 2006

Following correspondence with the source of this antibody I have determined that the concentration for the latest batch (#112817) of our anti human C4 binding protein is 10.8mg/ml. Please be aware that as the product is an IgG fraction, approximately ...

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Following correspondence with the source of this antibody I have determined that the concentration of the latest batch of ab8788 is 10.8mg/ml. Please be aware that as the product is an IgG fraction, approximately 10% of this will be specific antibody. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"