Mouse, Rat, Horse, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
A 14-22 amino acid region within synthetic peptide: SSIKKIADFL NSFDMSCRSR LATLNEKLTA LERRIEYIEA RVTKGETLT, corresponding to amino acids 27-49 of Human C3orf10
- WB: HepG2 cell lysate.
IHC-P: Human liver tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 2.5 µg/ml. Predicted molecular weight: 9 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use a concentration of 4 - 8 µg/ml.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
C3orf10 is involved in regulation of actin and microtubule organization and is part of a WAVE complex that activates the Arp2/3 complex.
- BRICK1, SCAR/WAVE actin nucleating complex subunit antibody
- BRICK1, SCAR/WAVE actin nucleating complex subunit, homolog antibody
- BRK1 antibody
Western blot - Anti-C3orf10 antibody (ab65445)
Anti-C3orf10 antibody (ab65445) at 2.5 µg/ml + HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 9 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3orf10 antibody (ab65445)
ab65445 at 4µg/ml, staining C3orf10 in paraffin embedded hepatocytes tissue from human liver (indicated by arrows) by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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