概述

  • 产品名称Anti-C3抗体
    参阅全部 C3 一抗
  • 描述
    兔多克隆抗体to C3
  • 特异性This polyclonal antibody detects a band approximately 120 kDa in Western blot under reducing conditions, corresponding to the C3 alpha chain.
  • 经测试应用适用于: IHC-FoFr, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Unfortunately, this information is considered to be commercially sensitive

性能

应用

Our Abpromise guarantee covers the use of ab11887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr 1/10.
WB 1/100. Detects a band of approximately 120 kDa under reducing conditions.

靶标

  • 功能C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • 组织特异性Plasma.
  • 疾病相关Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
    Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • 序列相似性Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • 翻译后修饰C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
    Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acylation stimulating protein cleavage product antibody
    • AHUS5 antibody
    • ARMD9 antibody
    • ASP antibody
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
    • C3 antibody
    • CO3_HUMAN antibody
    • Complement C3 antibody
    • Complement C3c alpha'' chain fragment 2 antibody
    • Complement component 3 antibody
    • Complement factor 3 antibody
    • CPAMD1 antibody
    • HEL S 62p antibody
    see all

Anti-C3 antibody 图像

  • Immunohistochemical analysis of rat retina tissue after bright continuous white light exposure, staining C3 with ab11887.

Anti-C3 antibody (ab11887)参考文献

This product has been referenced in:
  • Gao TT  et al. Complement factors C1q, C3 and C5b-9 in the posterior sclera of guinea pigs with negative lens-defocused myopia. Int J Ophthalmol 8:675-80 (2015). WB ; Guinea pig . Read more (PubMed: 26309860) »
  • Camporeale A  et al. STAT3 activity is necessary and sufficient for the development of immune-mediated myocarditis in mice and promotes progression to dilated cardiomyopathy. EMBO Mol Med 5:572-90 (2013). IHC-Fr ; Mouse . Read more (PubMed: 23460527) »

See all 8 Publications for this product

Product Wall

Thank you for contacting us.

The 120 kD band is the C3 alpha chain.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

---------------------...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (fibrotic liver)
Loading amount 20 µg
Specification fibrotic liver
Gel Running Conditions Reduced Denaturing (SDS boiling for 5 min)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Mr. Hao Wu

Verified customer

提交于 Nov 14 2012

Thank you for your enquiry. I have been in touch with the source of this antibody and according to the information that they have ab11887 recognizes whole protein C3 but does not recognize C3a and C3b; resulting from the cleavage of C3 into its alp...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"