Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
Synthetic peptide corresponding to a region within N terminal amino acids 37-86 DYDKKVKQAA KEKVRRRHTP APTRPRKPDL QVYLPRHRDV SAHPRNPDYE of Human C2orf68
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1/312500.
Three different isoforms have been described.
- Chromosome 2 open reading frame 68 antibody
- FLJ14112 antibody
- FLJ35653 antibody
- MGC131675 antibody
Western blot - C2orf68 antibody (ab81363)
Anti-C2orf68 antibody (ab81363) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
19 kDaObserved band size:
22 kDa (why is the actual band size different from the predicted?
)Additional bands at:
42 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 12%
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"