概述

  • 产品名称
    Anti-C2b抗体
  • 描述
    兔多克隆抗体to C2b
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide corresponding to Mouse C2b aa 100-200 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P21180
    (Peptide available as ab95402)

  • 阳性对照
    • This antibody gave a positive signal in Mouse liver tissue lysate.
  • 常规说明


    This antibody specifically recognises the C2b fragment of C2 (amino acids 21 - 243)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • Primary antibody说明
    This antibody specifically recognises the C2b fragment of C2 (amino acids 21 - 243)
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 84 kDa).

靶标

  • 相关性
    Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase.
  • 细胞定位
    Secreted
  • 数据库链接
  • 别名
    • C2 antibody
    • C2b antibody
    • CO2 antibody
    • complement C2 antibody
    • Complement C2b fragment antibody
    • complement component C2 antibody
    see all

Anti-C2b antibody 图像

  • Anti-C2b antibody (ab66999) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

实验方案

Anti-C2b antibody (ab66999)参考文献

ab66999 has not yet been referenced specifically in any publications.

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