Anti-C22orf28抗体(ab98231)

概述

  • 产品名称Anti-C22orf28抗体
    参阅全部 C22orf28 一抗
  • 描述
    兔多克隆抗体to C22orf28
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 107-156 (PEAVVSPGGV GFDINCGVRL LRTNLDESDV QPVKEQLAQA MFDHIPVGVG) of Human C22orf28 (NP_055121).

  • 阳性���照
    • HepG2 cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab98231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

靶标

Anti-C22orf28 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling C22orf28 with ab98231 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - C22orf28. Right - Merge.


  • Predicted band size : 55 kDa
    Western blot analysis of Jurkat cell lysate labeling C22orf28 with ab98231 at 1.0µg/ml.


  • Predicted band size : 55 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling C22orf28 with ab98231 at 1.0µg/ml.


  • Predicted band size : 55 kDa
    Western blot analysis of HeLa cell lysate labeling C22orf28 with ab98231 at 1.0µg/ml.
  • Anti-C22orf28 antibody (ab98231) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 55 kDa

Anti-C22orf28 antibody (ab98231)参考文献

ab98231 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS cells)
Loading amount 40 µg
Specification U2OS cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

提交于 Jan 02 2013

Thank you for your answer and sending the bovine sequence. Indeed, there is a 100% alingment with the immunogen and the chances are therefore very high that the antibody will crossreact. I am thus sending you here the testing discount code. Please see ...

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Thank you very much for your interest in ab98231.

Unfortunately we do not have any trial vials of our products. However if you would like to test this antibody, we can make you an interesting testing offer - please see below.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"