Anti-C22orf28抗体(ab118290)

概述

  • 产品名称Anti-C22orf28抗体
    参阅全部 C22orf28 一抗
  • 描述
    山羊多克隆抗体to C22orf28
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    QADPNKVSARAKKR

    with a Cysteine residue linker, corresponding to internal sequence amino acids 201-214 of Human C22orf28 (NP_055121.1)

  • 阳性对照
    • HeLa, Jurkat and K562 lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab118290 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab118290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P Use a concentration of 5 - 10 µg/ml.

靶标

  • 功能Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
  • 序列相似性Belongs to the RtcB family.
  • 细胞定位Nucleus. Cytoplasm. Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ankyrin repeat domain 54 antibody
    • Chromosome 22 open reading frame 28 antibody
    • DJ149A16.6 antibody
    • HSPC117 antibody
    • Hypothetical protein LOC51493 antibody
    • Novel protein HSPC117 antibody
    • OTTHUMP00000028639 antibody
    • RP1-149A16.6 antibody
    • rtcB antibody
    • RTCB_HUMAN antibody
    • tRNA-splicing ligase RtcB homolog antibody
    see all

Anti-C22orf28 antibody 图像

  • Staining of Human Testis by ab118290 (5μg/mL) in paraffin embedded tissue following stemaed antigen retieval with citrat buffer pH6, AP-Staining this shows nuclear and vicinity staining in spermatogonia.

  • Anti-C22orf28 antibody (ab118290) at 0.3 µg/ml + HeLa lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 55 kDa
    Observed band size : 55 kDa

Anti-C22orf28 antibody (ab118290)参考文献

ab118290 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS cells)
Loading amount 40 µg
Specification U2OS cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jan 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"