概述

  • 产品名称
    Anti-C22orf25抗体
    参阅全部 C22orf25 一抗
  • 描述
    兔多克隆抗体to C22orf25
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N-terminal amino acids 2-51 (CIIFFKFDPR PVSKNAYRLI LAANRDEFYS RPSKLADFWG NNNEILSGLD) of Human C22orf25 (NP_690870).

  • 阳性对照
    • Human fetal muscle lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab87576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-C22orf25 antibody 图像

  • Anti-C22orf25 antibody (ab87576) at 1 µg/ml + human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa

实验方案

Anti-C22orf25 antibody (ab87576)参考文献

This product has been referenced in:
  • Lalani SR  et al. Recurrent Muscle Weakness with Rhabdomyolysis, Metabolic Crises, and Cardiac Arrhythmia Due to Bi-allelic TANGO2 Mutations. Am J Hum Genet 98:347-57 (2016). Read more (PubMed: 26805781) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (muscle tissue)
Permeabilization
Yes - BSA5%- tween- triton1%
Specification
muscle tissue
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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提交于 Jun 29 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (THYROID GLAND)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CITRATE PH6
Permeabilization
No
Specification
THYROID GLAND
Blocking step
Novolink polymer detection system as blocking agent for 30 minute(s) · Concentration: 0.4% · Temperature: 25°C
Fixative
Formaldehyde
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提交于 May 31 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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