antigen, corresponding to N terminal amino acids 1-71 of Human C22orf15.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Information by UniProt
- C22orf15 antibody
- chromosome 22 open reading frame 15 antibody
- CV015_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-C22orf15 antibody (ab121549)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus. Recommended concentration of ab121549 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C22orf15 antibody (ab121549)
ab121549, at 1/200, staining C22orf15 in Paraffin-embedded Human liver tissue by Immunohistochemistry showing cytoplasmic positivity in hepatocytes.
has not yet been referenced specifically in any publications.
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