概述

  • 产品名称Anti-C21ORF62抗体
  • 描述
    兔多克隆抗体to C21ORF62
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (STNTLPTEYL AICGLKRLRI NMEAKHPFPE QSLLIHSGGD SDSREKPMWL) of Human C21ORF62 (NP_062542).

  • 阳性对照
    • 293T cell lysate. DU145 cell line (IF/ICC)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性The exact function of C21orf62 remains unknown.
  • 数据库链接
  • 别名
    • B37 antibody
    • C21orf120 antibody
    • Chromosome 21 open reading frame 62 antibody
    • Hypothetical protein LOC56245 antibody
    • PRED81 antibody
    • Uncharacterized protein C21orf62 antibody
    see all

Anti-C21ORF62 antibody 图像

  • Anti-C21ORF62 antibody (ab84746) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab84746 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84746, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-C21ORF62 antibody (ab84746)参考文献

ab84746 has not yet been referenced specifically in any publications.

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