概述

  • 产品名称Anti-C20orf19抗体
    参阅全部 C20orf19 一抗
  • 描述
    兔多克隆抗体to C20orf19
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 625 and the C-terminus (residue 673) of human C20orf19 (NP_060944.2)

  • 阳性对照
    • Whole cell lysates from Hela and 293T cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab72248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 75 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Centrosomal protein required for establishing a robust mitotic centrosome architecture that can endure the forces that converge on the centrosomes during spindle formation. Required for stabilizing the expanded pericentriolar material around the centriole.
  • 序列相似性Belongs to the kizuna family.
  • 翻译后修饰Phosphorylation at Thr-379 by PLK1 is not needed for centrosomal localization or pericentriolar material expansion but is indispensable for spindle-pole stabilization.
  • 细胞定位Cytoplasm > cytoskeleton > centrosome. Localizes to centrosomes throughout the cell cycle. After centrosome duplication, it usually remains associated only with the mother centrosome, containing the older mature centriole and particles surrounding it. During prophase, additional particles accumulate around both separating centrosomes. Does not accumulate at the microtubule minus ends, but instead localizes to the centrosomes and centrosome-surrounding area in a microtubule-independent and dependent manner, respectively.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Centrosomal protein kizuna antibody
    • DKFZP586H021 antibody
    • HT013 antibody
    • Kiz antibody
    • KIZ_HUMAN antibody
    • KIZUNA antibody
    • MGC102941 antibody
    • MGC141930 antibody
    • NCRNA00153 antibody
    • Plk1s1 antibody
    • Polo-like kinase 1 substrate 1 antibody
    • Uncharacterized protein C20orf19 antibody
    see all

Anti-C20orf19 antibody 图像

  • All lanes : Anti-C20orf19 antibody (ab72248) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 75 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa,70 kDa,80 kDa. We are unsure as to the identity of these extra bands.
  • Detection of C20orf19 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72248 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

Anti-C20orf19 antibody (ab72248)参考文献

ab72248 has not yet been referenced specifically in any publications.

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