概述

  • 产品名称Anti-C2抗体[6E3]
    参阅全部 C2 一抗
  • 描述
    小鼠单克隆抗体[6E3] to C2
  • 特异性Strong reaction is seen with human serum/plasma and no reaction is seen with serum from patients deficient in C2. ab17452 reacts with C2 and a subfraction of C2 believed to be C2b.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native complement component C2 protein, isolated from human plasma.

  • 表位Epitope specificity differs from that of ab17451 as determined by inhibition ELISA.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号6E3
  • 骨髓瘤x63-Ag8.653
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab17452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: 1/500.
    WB: 1/250. Predicted molecular weight: 102 kDa. ab17452 reacts with C2 under reducing and non-reducing conditions.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase.
    • 疾病相关Defects in C2 are the cause of complement component 2 deficiency (C2D) [MIM:217000]. A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections.
    • 序列相似性Belongs to the peptidase S1 family.
      Contains 1 peptidase S1 domain.
      Contains 3 Sushi (CCP/SCR) domains.
      Contains 1 VWFA domain.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ARMD14 antibody
      • C2 antibody
      • C3/C5 convertase antibody
      • CO2 antibody
      • CO2_HUMAN antibody
      • Complement C2 antibody
      • Complement C2a fragment antibody
      • complement component 2 antibody
      • DKFZp779M0311 antibody
      see all

    Anti-C2 antibody [6E3] (ab17452)参考文献

    This product has been referenced in:
    • Martini PG  et al. Recombinant human complement component C2 produced in a human cell line restores the classical complement pathway activity in-vitro: an alternative treatment for C2 deficiency diseases. BMC Immunol 11:43 (2010). Sandwich ELISA ; Human . Read more (PubMed: 20727163) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. I received the following information from the originator of these two antibodies: "The protocol we used to test these antibodies was a direct ELISA with a coat of C2, a layer with ab17451 (1:6500) or ab17452 (1:500), and det...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"