C1s overexpression 293T裂解液(全细胞) (ab94098)

概述

  • 产品名称C1s overexpression 293T裂解液(全细胞)
  • 描述
    C1s overexpression裂解液
  • 常规说明ab94098 is a 293T cell transfected lysate in which Human C1s has been transiently over-expressed using a pCMV-C1s plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • 存储溶液pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • 研究领域
  • 背景Function: C1s B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4. Disease: Defects in C1S are the cause of complement component C1s deficiency (C1SD) [MIM:613783]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. Similarity: Belongs to the peptidase S1 family. Contains 2 CUB domains. Contains 1 EGF-like domain. Contains 1 peptidase S1 domain. Contains 2 Sushi (CCP/SCR) domains. PTM: The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

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应用

Our Abpromise guarantee covers the use of ab94098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

C1s overexpression 293T lysate (whole cell) 图像

  • ab94098 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-C1s antibody (ab89598) at 1/500 dilution

    Lane 1 : C1s overexpression 293T lysate (whole cell) (ab94098)
    Lane 2 : 293T Non Transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

C1s overexpression 293T lysate (whole cell) (ab94098)参考文献

ab94098 has not yet been referenced specifically in any publications.

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