概述

  • 产品名称Anti-C1s抗体[M81]
    参阅全部 C1s 一抗
  • 描述
    小鼠单克隆抗体[M81] to C1s
  • 特异性ab81707 reacts around the binding site of C1s and reacts with both active and inactive C1s.
  • 经测试应用适用于: ICC/IF, WB, IP, ELISA, IHC-P, IHC-Fr, Functional Studies, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 表位ab81707 reacts with an epitope on human C protein activated C1s, a subcomponent of the first component of C (C1). The epitope recognized by ab81707 is domain IV and/or V of the gamma domain of activated C1s.
  • 阳性对照
    • IHC-P: Human liver melanoma cells.

性能

应用

Our Abpromise guarantee covers the use of ab81707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/10 - 1/50. Predicted molecular weight: 55 kDa.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/10 - 1/50.
Functional Studies Use at an assay dependent concentration.
Flow Cyt 1/10 - 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能C1s B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.
  • 疾病相关Defects in C1S are the cause of complement component C1s deficiency (C1SD) [MIM:613783]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 1 EGF-like domain.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • 翻译后修饰The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Basic proline rich peptide IB 1 antibody
    • C1 esterase antibody
    • C1S antibody
    • C1S_HUMAN antibody
    • Complement C1s subcomponent antibody
    • Complement C1s subcomponent heavy chain antibody
    • Complement C1s subcomponent light chain antibody
    • Complement component 1 s subcomponent antibody
    • Complement component 1 subcomponent s antibody
    • FLJ44757 antibody
    see all

Anti-C1s antibody [M81] 图像

  • ab81707, at a dilution of 1/50, staining paraffin embedded human liver melanoma cells by Immunohistochemistry. Insert is isotype control.

Anti-C1s antibody [M81] (ab81707)参考文献

This product has been referenced in:
  • Matsumoto M  et al. Probing the C4-binding site on C1s with monoclonal antibodies. Evidence for a C4/C4b-binding site on the gamma-domain. J Immunol 142:2743-50 (1989). Read more (PubMed: 2522968) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (liver)
Specification liver
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer for 8 min
Permeabilization No
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Abcam user community

Verified customer

提交于 May 02 2012

Thank you for your patience.

I have received the specific IHC-P protocol for the image on the datasheet:


Short IHC-P protocol for antibody ab81707 to C1 Inactivator [M81]


Tissue used: liver melanoma.
Resul...

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Thank you for contacting us.

Please see below for the IHC-P protocol. I am also checking with the lab regarding the details for the IHC image on the datasheet and will let you know what I find out. Thank you foryour patience.


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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"