概述

  • 产品名称Anti-C1s抗体
    参阅全部 C1s 一抗
  • 描述
    兔多克隆抗体to C1s
  • 特异性ab72651 detects endogenous levels of fragment of activated C1s resulting from cleavage adjacent to Arg437.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from human C1s.

  • 阳性对照
    • Extracts from 293 cells treated with etoposide (25uM, 1hour) and HeLa cells treated with etoposide (25uM, 24hours).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: 1/20000.
    WB: 1/500 - 1/1000. Detects bands of approximately 75 & 51 kDa (predicted molecular weight: 77 & 51 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能C1s B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.
    • 疾病相关Defects in C1S are the cause of complement component C1s deficiency (C1SD) [MIM:613783]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
    • 序列相似性Belongs to the peptidase S1 family.
      Contains 2 CUB domains.
      Contains 1 EGF-like domain.
      Contains 1 peptidase S1 domain.
      Contains 2 Sushi (CCP/SCR) domains.
    • 翻译后修饰The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Basic proline rich peptide IB 1 antibody
      • C1 esterase antibody
      • C1S antibody
      • C1S_HUMAN antibody
      • Complement C1s subcomponent antibody
      • Complement C1s subcomponent heavy chain antibody
      • Complement C1s subcomponent light chain antibody
      • Complement component 1 s subcomponent antibody
      • Complement component 1 subcomponent s antibody
      • FLJ44757 antibody
      see all

    Anti-C1s antibody 图像

    • All lanes : Anti-C1s antibody (ab72651) at 1/500 dilution

      Lane 1 : Extracts from 293 cells treated with etoposide (25uM, 1hour)
      Lane 2 : Extracts from HeLa cells treated with etoposide (25uM, 24hours)
      Lane 3 : Extracts from HeLa cells treated with etoposide (25uM, 24hours) with Cl-peptide at 5 µg

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 77 , 51 kDa
      Observed band size : 51,75 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

    Anti-C1s antibody (ab72651)参考文献

    ab72651 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab72651.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"