ab72648 detects a fragment (light chain) of activated C1r resulting from cleavage adjacent to Ile464.
Synthetic peptide derived from human C1r.
Extracts from 293 cells treated with etoposide (25uM, 1hour) and A549 cells treated with etoposide (25uM, 24hours).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.
Belongs to the peptidase S1 family.
Contains 2 CUB domains. Contains 1 EGF-like domain. Contains 1 peptidase S1 domain. Contains 2 Sushi (CCP/SCR) domains.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Information by UniProt
C1 R antibody
Western blot - C1r antibody (ab72648)
All lanes : Anti-C1r antibody (ab72648) at 1/500 dilution Lane 1 : Extracts from 293 cells treated
with etoposide (25uM, 1hour) Lane 2 : Extracts from A549 cells treated with
etoposide (25uM, 24hours) Lane 3 : Extracts from 293 cells treated
with etoposide (25uM, 1hour) with immunising peptide at 5 µg Lysates/proteins at 5 µg per lane. Predicted band size : 29 kDa Observed band size : 29 kDa Additional bands at : 19 kDa,40 kDa,60 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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