Anti-C1QB抗体(ab90181)

概述

  • 产品名称Anti-C1QB抗体
    参阅全部 C1QB 一抗
  • 描述
    兔多克隆抗体to C1QB
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (GPKGESGDYK ATQKIAFSAT RTINVPLRRD QTIRFDHVIT NMNNNYEPRS) of Human C1QB (NP_000482).

  • 阳性对照
    • Human fetal stomach lysate

性能

应用

Our Abpromise guarantee covers the use of ab90181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF 1/600.

靶标

Anti-C1QB antibody 图像

  • ab90181 staining C1QB in Rat brain tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Tween 29 and blocked with 2% BSA for 20 minutes at 21°C. Samples were incubated with primary antibody (1/600 in PBS-T) for 12 hours. An Alexa Fluor®594-conjugated Goat anti-rabbit polyclonal(1/1000) was used as the secondary antibody.

    See Abreview

  • Anti-C1QB antibody (ab90181) at 1 µg/ml + Human fetal stomach lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 26 kDa

Anti-C1QB antibody (ab90181)参考文献

ab90181 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (brain tissue)
Specification brain tissue
Fixative Paraformaldehyde
Permeabilization Yes - TWEEN-20
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Apr 12 2013

For ab112521: DISCOUNT CODE:***

For ab7206: DISCOUNT CODE: ***

For ab79044: DISCOUNT CODE: ***

For ab133173: DISCOUNT CODE: ***

For ab43773: DISCOUNT CODE: ***

For ab90181: DISCOUNT CODE:***

E...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"