Anti-C1QA抗体(ab64632)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to C1QA
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to Mouse C1QA aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab91203)

  • 阳性对照
    • This antibody gave a positive signal in Mouse blood plasma tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab64632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 26 kDa).

靶标

  • 功能
    C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
  • 疾病相关
    Defects in C1QA are the cause of complement component C1qA deficiency (C1QAD) [MIM:120550]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
  • 序列相似性
    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • 翻译后修饰
    O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C1qa antibody
    • C1QA_HUMAN antibody
    • Complement C1q subcomponent subunit A antibody
    • Complement component 1 q subcomponent A chain antibody
    • Complement component 1 q subcomponent alpha polypeptide antibody
    • Complement component C1q A chain antibody
    see all

Anti-C1QA antibody 图像

  • Anti-C1QA antibody (ab64632) at 1 µg/ml + Plasma (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 36 + 33 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 minutesThe 33 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Mouse Complement C1q subcomponent subunit A precursor. C1q also contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

实验方案

Anti-C1QA antibody (ab64632)参考文献

ab64632 has not yet been referenced specifically in any publications.

Product Wall


The C1q antibody ab64632 is specific for subunit A.

Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Rat Cell lysate - whole cell (Brain tissue lysate)
Specification
Brain tissue lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Dec 24 2013

Thank you for contacting us. None of our antibodies have been validated for in vivo or diagnostic use. This C1q antibody in particular (ab64632) contains sodium azide, which can be very toxic in vivo. I hope this helps, please let me kno...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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