概述

  • 产品名称Anti-C1q抗体[JL-1]
    参阅全部 C1q 一抗
  • 描述
    小鼠单克隆抗体[JL-1] to C1q
  • 经测试应用适用于: ICC/IF, ELISA, Functional Studies, WB, IHC-Fr, IHC-FoFr, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Purified mouse C1q

  • 表位The monoclonal antibody JL-1is reactive with the collagen-like region (CLR).
  • 阳性对照
    • Spleen and kidney tissue of wild-type mice.
  • 常规说明The antibody has been generated by immunization of C1q-/- C57BL/6 mice with purified mouse C1q.

性能

应用

Our Abpromise guarantee covers the use of ab71940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. JL-1 was administered to mice resulting in depletion of circulating C1q, glomerular deposition of C1q and induction of anti-C1q autoantibodies in susceptible mice. As a negative control an isotype matched monoclonal antibody was used.
WB 1/50. Predicted molecular weight: 26 kDa.
IHC-Fr 1/50.
IHC-FoFr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

靶标

Anti-C1q antibody [JL-1] 图像

  • ab71940 staining C1q in mouse liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices, permeablized with 0.1 M PBS with 3% Triton X and blocked with 10% serum for 60 minutes at 24°C. The sample was incubated with primary antibody (1/100 in 0.3M PBST with 10% donkey serum) at 4°C for 24 hours. An Alexa Fluor® 488-conjugated donkey monoclonal (1/1000) was used as the secondary antibody.

  • ab71940 staining C1q in rat spleen tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde, blocked with 10% serum for 30 minutes at 24°C then incubated with ab71940 at a 1/50 dilution for 16 hours at 4°C. The seconday used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/1000 dilution.

    See Abreview

Anti-C1q antibody [JL-1] (ab71940)参考文献

This product has been referenced in:
  • Liu XR  et al. Transcriptomic Insights into the Response of Placenta and Decidua Basalis to the CpG Oligodeoxynucleotide Stimulation in Non-Obese Diabetic Mice and Wild-Type Controls. Int J Mol Sci 17:N/A (2016). Mouse . Read more (PubMed: 27527166) »
  • Gao TT  et al. Complement factors C1q, C3 and C5b-9 in the posterior sclera of guinea pigs with negative lens-defocused myopia. Int J Ophthalmol 8:675-80 (2015). WB ; Guinea pig . Read more (PubMed: 26309860) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Liver)
Permeabilization Yes - 0.1 M PBS with 3% Triton X
Specification Liver
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Feb 12 2016

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Mouse Tissue sections (spleen)
Specification spleen
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 28 2014

Application Immunocytochemistry
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Human Cell (Kelly Cell line)
Specification Kelly Cell line
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 28 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10µg/mL · Temperature: 24°C
Sample Human Tissue sections (Retina)
Specification Retina
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 28 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Rat Cell (B50)
Specification B50
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 28 2014

Thank you for contacting us. Please find attached the publication that shows a lot of the characterization work. We can confirm that the antibody is specific for mouse C1q and does not give aspecific staining in mouse. The antibody has also been checke...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Rat Cell (33B Cell line)
Specification 33B Cell line
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Sep 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Kelly Neuroblastoma cell line)
Specification Kelly Neuroblastoma cell line
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Sep 26 2012

Thank you for contacting regarding purified mouse C1q.
Unfortunately we are unable to offer this product for sale at this time. As I had mentioned in previous correspondences we do have a purified human protein and mouse peptide available should yo...

Read More

Thank you for your reply.

I have contacted the lab to see if this is available and will let you know as soon as I receive this information.

I hope that this information is helpful. Please let me know if you have any questions or there...

Read More

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"