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Purified mouse C1q
Our Abpromise guarantee covers the use of ab71940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration. JL-1 was administered to mice resulting in depletion of circulating C1q, glomerular deposition of C1q and induction of anti-C1q autoantibodies in susceptible mice. As a negative control an isotype matched monoclonal antibody was used.|
|WB||1/50. Predicted molecular weight: 26 kDa.|
|IHC-FoFr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
ab71940 staining C1q in mouse liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices, permeablized with 0.1 M PBS with 3% Triton X and blocked with 10% serum for 60 minutes at 24°C. The sample was incubated with primary antibody (1/100 in 0.3M PBST with 10% donkey serum) at 4°C for 24 hours. An Alexa Fluor® 488-conjugated donkey monoclonal (1/1000) was used as the secondary antibody.