Anti-C1q抗体(ab8779)

概述

  • 产品名称Anti-C1q抗体
    参阅全部 C1q 一抗
  • 描述
    羊多克隆抗体to C1q
  • 特异性This product gives a single arc when tested by IEP against fresh human plasma. Identity has been confirmed by double diffusion (Ouchterlony) vs fresh human plasma and a known anti-human C1q.
  • 经测试应用适用于: Double Immunodiffusion, RID, Immunoelectrophoresismore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human C1q prepared form human plasma. Greater than 95% purity by SDS-PAGE.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Glycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Antiserum is prepared by immunisation of sheep with human C1q and, if necessary, adsorbed to monospecificity by use of solid-phase adsorbents. Following adsorption an immunoglobulin fraction is produced by ion-exchange chromatography. Zone electrophoresis is performed to ensure that only a gamma-mobility component is present. The productis 0.2µm filtered.
  • 克隆多克隆
  • 同种型IgG
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Double Immunodiffusion
RID
Immunoelectrophoresis
Rocket Immunoelectrophoresis
  • 应用说明RID and Rocket IEP: 0.1µL antiserum/cm2 in gel vs 20µL fresh plasma, NT – 1/2
    Double Diffusion: 10µL antiserum vs 20µL fresh plasma
    Second Antibody: 100µL antiserum vs 10µL fresh plasma
    The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.
    Results can be enhanced by washing completed agarose gels in saline, drying and finally stainingwith a protein stain such as coomassie blue.
    Suitability for use in immunohistochemical procedures, enzyme-linked immunosorbent assays and Western blot has not been assessed butuse in such assays should not necessarily be excluded.
  • 靶标

    • 功能C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
    • 疾病相关Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
    • 序列相似性Contains 1 C1q domain.
      Contains 1 collagen-like domain.
    • 翻译后修饰O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C1qa antibody
      • C1QA_HUMAN antibody
      • C1QB antibody
      • C1QC antibody
      • Complement C1q subcomponent subunit A antibody
      • Complement C1q subcomponent subunit B antibody
      • Complement C1q subcomponent subunit C antibody
      • Complement component 1 q subcomponent alpha polypeptide antibody
      • Complement component C1q A chain antibody
      • Complement component C1q B chain antibody
      • Complement component C1q C chain antibody
      see all

    Anti-C1q antibody (ab8779)参考文献

    ab8779 has not yet been referenced specifically in any publications.

    Product Wall

    - We do not know whether it recognizes the globular head of the protein. Here is further information regarding the concentration/purification
    - The concentration of the current lot is 14.98mg/ml. The concentration is lot specific but the process f...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"