概述

  • 产品名称
    Anti-C1q抗体[9A7]
    参阅全部 C1q 一抗
  • 描述
    小鼠单克隆抗体[9A7] to C1q
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    C1q protein purified from Human plasma

  • 阳性对照
    • K562, Jurkat, C6 and NIH3T3 cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab71089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

图片

  • All lanes : Anti-C1q antibody [9A7] (ab71089) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Jurkat T cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : NIH3T3 cell lysate


    Predicted band size : 26 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ab71089 (1µg/ml) staining C1q in human stomach using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of of the epithelium.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

文献

This product has been referenced in:
  • Lai YC  et al. Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection. Sci Rep 7:6975 (2017). Read more (PubMed: 28765561) »
  • Lui H  et al. Progranulin Deficiency Promotes Circuit-Specific Synaptic Pruning by Microglia via Complement Activation. Cell 165:921-35 (2016). Flow Cyt ; Mouse . Read more (PubMed: 27114033) »

See all 6 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Human Serum (Serum)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Serum
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 28°C
Username

任弘 東岸

Verified customer

提交于 Aug 06 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (colon)
Specification
colon
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana - cc1mild
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Feb 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (Brain)
Loading amount
20 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Thermo SuperBlock Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Username

Dr. Lampros Panagis

Verified customer

提交于 Nov 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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