Rabbit, Guinea pig, Cow, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region within amino acids 100-150 of Human C1orf80 (NP_073742.2).
Jurkat, HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
Immunogen affinity purified
ab117780 was affinity purified using an epitope specific to C1orf80 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 35 kDa.
Use at 2-10 µg/mg of lysate.
Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling.
Widely expressed in adult tissues, with highest expression in the heart and skeletal muscle.
Belongs to the AIDA family.
Information by UniProt
Western blot - Anti-C1orf80 antibody (ab117780)
All lanes : Anti-C1orf80 antibody (ab117780) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : Jurkat whole cell lysate at 50 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 35 kDa Exposure time : 30 seconds
Immunoprecipitation - Anti-C1orf80 antibody (ab117780)
Detection of C1orf80 by Western Blot of Immunprecipitate.
ab117780 at 0.4µg/ml staining C1orf80 in HeLa whole cell lysate immunoprecipitated using ab117780 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"