antigen sequence: QFSIHASSNV NKQEENGVFI KTTDDTTTDN YIAQGKRKSN EMITNLGKKQ KTDVSTEHPP FYYNIHRHSI KGMNMPKLKK FLCYLSQAGF RVSRTHFD, corresponding to amino acids 574-671 of Human C1orf25.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May play a role in motor coordination and exploratory behavior.
Belongs to the TRM1 family.
Contains 1 C2H2-type zinc finger.
Information by UniProt
Chromosome 1 open reading frame 25 antibody
Hypothetical protein FLJ90512 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C1orf25 antibody (ab121883)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and nucleoli. Recommended concentration of ab121883 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1orf25 antibody (ab121883)
ab121883 at 1/200 dilution staining C1orf25 in Paraffin-embedded Human Placenta tissue by Immunohistochemistry.
Western blot-Anti-C1orf25 antibody(ab121883)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
has not yet been referenced specifically in any publications.
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