概述

  • 产品名称
    Anti-C1orf228抗体
    参阅全部 C1orf228 一抗
  • 描述
    兔多克隆抗体to C1orf228
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: VLGTMHLEVQ YEAIELIKDL VGYDVRQALL KGLVALLIPS VKEISKLQAK ILSDPSVLQL TPSLPMF, corresponding to amino acids 230-296 of Human C1orf228.

  • 阳性对照
    • Human small intestine tissue; RT-4 cell lysate; Human Liver lysate
  • 常规说明


    ab122232 is mono-specific.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • Primary antibody说明
    ab122232 is mono-specific.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250 - 1/500.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

图片

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122232 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122232, at 1/20 dilution, staining C1orf228 in paraffin-embedded Human small intestine by Immunohistochemistry.
  • All lanes : Anti-C1orf228 antibody (ab122232) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

文献

ab122232 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab122232.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册