antigen sequence, corresponding to amino acids 6-117 of Human C1orf21. (Q9H246)
Human cerebral cortex tissue; C1orf21 over-expression HEK293T lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/800. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Anti-C1orf21 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-C1orf21 antibody (ab121891)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab121891 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1orf21 antibody (ab121891)
ab121891 at 1/800 dilution staining C1orf21 in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
Western blot - Anti-C1orf21 antibody (ab121891)
All lanes : Anti-C1orf21 antibody (ab121891) at 1/250 dilution Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Anti-C1orf21 antibody (ab121891)参考文献
has not yet been referenced specifically in any publications.
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