antigen, corresponding to amino acids 127-240 of Human C1orf174 (Q8IYL3).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Belongs to the UPF0688 family.
Information by UniProt
- C1orf174 antibody
- CA174_HUMAN antibody
- Chromosome 1 open reading frame 174 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C1orf174 antibody (ab121278)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclei. Recommended concentration of ab121278 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1orf174 antibody (ab121278)
ab121278, at 1/50, staining C1orf174 in glandular cells of paraffin embedded Human stomach tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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