概述

  • 产品名称Anti-C1orf135抗体
    参阅全部 C1orf135 一抗
  • 描述
    兔多克隆抗体to C1orf135
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    amino acids 11-93 (CGVWLDAAAL KRRKVQTHLI KPGTKMLTLL PGERKANIYF TQRRAPSTGI HQRSIASFFT LQPGKTNGSD QTSVSSHTES QIN) of Human C1orf135 (Q9H7T9).

  • 阳性对照
    • HEK293T transfected lysate. Human bone marrow tissue.

性能

应用

Our Abpromise guarantee covers the use of ab122014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-C1orf135 antibody 图像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in centrosome. Recommended concentration of ab122014 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-C1orf135 antibody (ab122014) at 1/250 dilution

    Lane 1 : Vector only transfected HEK293T lysate
    Lane 2 : C1orf135 (co-expressed with a C-terminal myc-DDK tag ~3.1 kDa) transfected HEK293T lysate

    Developed using the ECL technique
  • ab122014, at a 1/50 dilution, staining C1orf135 in paraffin-embedded Human bone marrow, by Immunohistochemistry.

Anti-C1orf135 antibody (ab122014)参考文献

ab122014 has not yet been referenced specifically in any publications.

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