antigen sequence: SHQNVLSNYF PRVSFANQKA FRGVNGSPRI SVTVGNIPKN SVSSSSQRRV SSSKISLRNS SKVTESASVM PSQDVSGSED T, corresponding to internal sequence amino acids 323-403 of Human C1orf124.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-C1orf124 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-C1orf124 antibody (ab121853)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab121853 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1orf124 antibody (ab121853)
ab121853, at a 1/325 dilution, staining C1orf124 in Purkinje cells of paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
Anti-C1orf124 antibody (ab121853)参考文献
has not yet been referenced specifically in any publications.
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