antigen sequence: IALQLKATLE NITNLRPVGE DFRWYLKMKC GNCGEISDKW QYIRLMDSVA LKGGRGSASM VQKCKLCARE, corresponding to amino acids 4-73 of Human C1orf123. (Q9NWV4)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Belongs to the UPF0587 family.
Information by UniProt
Chromosome 1 open reading frame 123 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C1orf123 antibody (ab122865)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122865 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-C1orf123 antibody (ab122865)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1orf123 antibody (ab122865)
ab122865 at 1/20 dilution staining C1orf123 in paraffin-embedded Human liver tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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