概述

  • 产品名称
    Anti-C1orf123抗体
  • 描述
    兔多克隆抗体to C1orf123
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: IALQLKATLE NITNLRPVGE DFRWYLKMKC GNCGEISDKW QYIRLMDSVA LKGGRGSASM VQKCKLCARE, corresponding to amino acids 4-73 of Human C1orf123. (Q9NWV4)

  • 阳性对照
    • Human liver tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

图片

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122865 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
  • ab122865 at 1/20 dilution staining C1orf123 in paraffin-embedded Human liver tissue by Immunohistochemistry.

文献

ab122865 has not yet been referenced specifically in any publications.

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