Anti-C1orf106抗体(ab121945)

概述

  • 产品名称Anti-C1orf106抗体
    参阅全部 C1orf106 一抗
  • 描述
    兔多克隆抗体to C1orf106
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: PPQTLEGLQP TGPEAGSPER APVQNSPWKE TSLDHPYEKP RKSSEPWSES SSPATTPQDG PSASSLWLLE PASYHVVPIR G, corresponding to amino acids 297 - 377 of Human C1orf106 (UniProt ID: Q3KP66).

  • 阳性对照
    • Human stomach tissue
      RT-4 lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab121945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/500 - 1/1000.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-C1orf106 antibody 图像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm and golgi apparatus. Recommended concentration of ab121945 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-C1orf106 antibody (ab121945) at 1/250 dilution

    Lane 1 : RT-4 lysate
    Lane 2 : U-251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate
  • ab121945, at a 1/85 dilution, staining C1orf106 in paraffin-embedded Human stomach tissue by Immunohistochemistry.

Anti-C1orf106 antibody (ab121945)参考文献

ab121945 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (macrophage)
Specification macrophage
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Dec 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"